77 grados Kelvin

LA SELECCIÓN DE RAZAS ES EL PRIVILEGIO … LA CONSERVACIÓN ex situ ES LA REGALÍA.

GLYCEROL AND SUCROSE ON FROZEN-THAWED BOVINE EMBRYOS.

Bovine embryos were collected on Day 7-8 after artificial insemination and frozen with I-step (10%) or 4-step (0, 3.3, 8.3 and 10%) glycerol with or without sucrose (0.5 M) by slow-rate freezing protocol. After IS days in liquid nitrogen, embryos were thawed and glycerol was removed by step or in straw. dilution.
Results of this study showed no significant differences in embryo quality and damage of zona pellucida (ZP) (p>0.05) after freezing with 1- or 4-step glycerol. The presence of sucrose improved embryo quality and reduced damage of ZP (p<0.05). The damage of ZP was reduced (P<0.05) when glycerol was removed by step dilution.

FUENTE

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