In the horse, the isolation percentage of Mesenchymal stem cells (MSCs) from peripheral blood is very low and bone marrow is currently the main source of equine MSCs [25-29]. There are no peer-reviewed reports of equine adipose tissue derived stem cells although commercial marketing of so-called equine adipose tissue derived stem cell therapy is taken place today . There appears to be no published studies on equine cord blood stem cells, hematopoietic or mesenchymal, although commercial storage of equine cord blood for possible future autologous stem cell transplantations is available . Mesenchymal stem cells isolation from thawed human cord blood is associated with significantly
reduce success [31,32] and the future isolation percentage of MSCs from cryopreserved equine cord blood is therefore expectedly low.
Isolation of MSCs cells from equine umbilical cord blood could provide a non-invasive source of stem cells with potentially superior cellular characteristic to other equine stem cells with regard to immune tolerance, proliferative potential and differentiation potency. This study reports the isolation of cryopreservation and thawing tolerant MSCs from fresh equine cord blood with in vitro differentiation potential towards the osteogenic, chondrogenic and adipogenic cell lineages.